Detection of apoptosis by flow cytometry of cells simultaneously stained for intracellular pH (carboxy SNARF‐1) and membrane permeability (Hoechst 33342)
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چکیده
منابع مشابه
assessment of different permeabilization methods of minimizing damage to the adherent cells for detection of intracellular rna by flow cytometry
background: various fixation and permeabilization techniques have been developed for detection of intracellular antigens by flow cytometry; however, there are few studies using flow cytometry to detect the frequency of intracellular nucleic acids, particularly rna. we tested six different permeabilization methods in order to gain access to a high quality method with minimal damage to intracellu...
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ژورنال
عنوان ژورنال: Cytometry
سال: 1996
ISSN: 0196-4763,1097-0320
DOI: 10.1002/(sici)1097-0320(19961201)25:4<349::aid-cyto6>3.3.co;2-x